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Part:BBa_K648107:Design

Designed by: Alex Bina   Group: iGEM11_Penn_State   (2011-10-23)


RecA1 with mutations of Arg243 and Lys286


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Remove restriction sites and all recombinant activity.


Source

genome sequence from DH10B strain of Escherichia coli bacteria.

References